Chemical molecules are attached to the probe and that will enable the detection of this after a process of hybridization. There are many types of reporter molecules: radioactive (32P, 35S), affinity (Biotin, Digoxigenin ...), enzymatic (phosphatase, peroxidase ...) and chemiluminescent (esters of acridine).

What factors affect the sensitivity and specificity of the hybridization reaction? (more...)