Archive for the ‘RCAT’ Category
RCATTM Technology Details
August 27th, 2009
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Ultrasensitive, Multiplexed Diagnostics
Unmet Needs Addressed
New technologies are required that enable:
- Earlier detection of disease
- The potential to cut costs by reductions in labor, lower repeat volumes, and consolidation of tests on fewer testing platforms
- Improvements in turnaround times, convenience, and
RCATTM For Cells and Tissues
Immunohistochemistry (IHC) and flow cytometry are highly respected technologies for the diagnosis of cancer and infectious disease. They are widely used by pathologists and increasingly used by large pharmaceutical and biotechnology companies to assist in target validation and the discovery of new drugs.
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RCATTM on Beads
Microspheres, also known as latex beads, polystyrene beads, and microbeads
are a proven solid-phase format for development of assays used in both research
and clinical diagnostics.
Key advantages to bead format assays are:
- Ease of manufacture
- Faster assays; near–fluid-phase binding kinetics
- Increased sensitivity; increased surface allows more antigen, antibody,
or DNA to be included in the assay - Easily automated assay formats
RCATTM Technology Overview
To diagnose disease, molecules characteristic of that disease must be studied, such as the DNA of a bacteria or virus, a protein produced by cancer cells, antibodies to an infection, or the sequence of a gene mutation. These may be present at very low, often undetectable levels.
RCAT enables either the target or a signal attached to the target to be amplified in a way that allows detection of important biological markers that until now have been undetectable.
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RCATTM Advantages
Rolling Circle Amplification Technology (RCAT)
is a proprietary amplification technology that offers multiple advantages
to help meet the emerging needs of life sciences industries.
RCAT: First-time performance advantages
Expanded multiplexing
for protein microarrays
RCAT enables an unprecedented combination
of sensitivity and multiplexing advantages for protein microarrays. This
allows researchers for the first time to take advantage of the efficiency,
throughput, and utility of microarrays for protein detection and measurement.
Tags: RCATTM Technology Details
RCAT is a highly sensitive and efficient amplification method that has been configured in a number of formats to address a broad range of applications.
Linear RCAT (L-RCAT)
One end of a DNA probe is joined to the desired target.
The other end is hybridized to a generic DNA circle.
An enzyme catalyst is added. Starting at the probe for the circle, the enzyme catalyst makes a copy of the DNA circle, returns to the starting point and continues to copy the circle.
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RCATTM for Microarrays
The microarray is currently the format of choice for many approaches to
genomics and pharmacogenomics and holds considerable promise as a platform
for diagnostics and proteomics.
Key advantages of microarrays are:
- Massive multiplexing,
- Multipurpose applicability,
- Minute reagent consumption.