Archive for the ‘Diagnostics’ Category
Methods for DNA sequencing
August 30th, 2009
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Liquid gold organisms
Not many people get organic substances in pure form (or in the form of homogeneous mixtures) are already in antiquity. Fats and oils, no doubt, are among their number. Therefore, their names are in all languages simple native, although the chemists call them also lipids (from the Greek "liposome" - fat). To detect them was easy because they occur in all living organisms, and often in large numbers. Nature took care of animals and plants, providing them with supplies of fuel for cold and hungry days. Animals put this fuel, mainly around the internal organs and under the skin to fat deposits at the same time served as a shock-absorbing cushion shocks and heat insulator. OtherHuman skin analysis
Human skin is a very complicated structure, constructed from various types of chemicals. It is an integrated system with interacting with each other chemical components. Human skin in 69-73% of water.
Different layers of the skin vary considerably in its content: the share of the epidermis is about 10%, papillary layer - 71-72%. net dermis - about 61%. Normal cells activity is under constant ionic composition and pH of body fluids. In the water, readily soluble chemical compounds containing polar groups and capable of entering into the dipole-dipole interactions with water molecules or form with hydrogen bonds (-OH,-NH, C = 0): non-polar hydrocarbon molecules poorly or not soluble in it.
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Gene-Based Diagnostics
Gene-Based Diagnostics Through RCAT™—Fast and Accurate
Gene-based diagnostic tests are the fastest growing segment of the in vitro diagnostic market. They use the tools of molecular biology to identify nucleotide substitutions, deletions, or insertions in genes of medical interest. These tests provide several advantages over immunodiagnostic tests. For example, an immunodiagnostic HIV test, the current industry standard, detects the presence of HIV antibodies in blood. A person’s immune system can take several weeks to produce antibodies after an infection so there can be a long window of time between infection and detection. Gene-based diagnostic tests can measure the RNA of the HIV virus directly, greatly reducing the time it takes to diagnose the disease.
RCAT™—Immunodiagnostics
Proteins and small molecules are commonly measured indirectly in diagnostics, through the use of antibodies (”immunodiagnostics”). Immunodiagnostic testing is the current “gold standard” for many diagnostic determinations. The application of RCAT™ in immunodiagnostics offers a significant opportunity for increasing the sensitivity of these tests.
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Ultrasensitive, Multiplexed Diagnostics
RCATTM technology has significant
advantages for the rapidly growing molecular diagnostic market as well
as the larger, more established market for immunodiagnostic tests.
Unmet Needs Addressed
New technologies are required that enable:
- Earlier detection of disease
- The potential to cut costs by reductions in labor, lower repeat volumes, and consolidation of tests on fewer testing platforms
- Improvements in turnaround times, convenience, and portability
- New proprietary tests for improved profitability
- An ability to address new markets such as pharmacogenomic testing
The First Effective Whole Genome Amplification
Unlimited DNA from a few cells.
The
growing trend to study disease and drug response at the molecular level
has focused attention on DNA as a precious resource. Multiple Displacement
Amplification (MDA) technology developed by MSI enables the first effective
whole genome amplification method. The MDA method is a quantum leap over
other currently available whole genome amplification methods, which are
based on PCR
The MSI MDA method rapidly amplifies the genome with comprehensive loci
coverage and minimal bias between any loci, yielding 10+kb fragments in
a simple, scalable reaction.
Tags: Proteomics
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DNA at end of reaction.
Most amplified strands are converted to double-stranded DNA at end of reaction.
stranded template for on-going priming
After initial priming, an exponential cascade of branched amplification generates single-stranded template for on-going priming.
