The First Effective Whole Genome Amplification.
Unlimited DNA from a few cells.

The growing trend to study disease and drug response at the molecular level has focused attention on DNA as a precious resource. Multiple Displacement Amplification (MDA) technology developed by MSI enables the first effective whole genome amplification method. The MDA method is a quantum leap over other currently available whole genome amplification methods, which are based on PCR (MDA Technology Overview). The MSI MDA method rapidly amplifies the genome with comprehensive loci coverage and minimal bias between any loci, yielding 10+kb fragments in a simple, scalable reaction.

The MSI MDA technology platform for whole genome amplification can transform the current practice of sample preparation and sample archiving for genetic testing.
  • Comprehensive coverage and 10+kb fragments expand applications
  • Increased accuracy provides amplified material true to the original
  • Simplified method eliminates sample preparation step
  • Provides assay-ready DNA
The MDA technology platform for whole genome amplification enables:
  • A virtually unlimited number of tests per sample
  • Use of smaller samples, such as buccal swabs, mouthwash, and finger prick
  • Use of DNA from alternative sources, such as biopsies and needle aspirates
  • An increase in the practice of archiving DNA for additional testing or re-testing
  • Amplification directly from whole blood and cells

Optimal coverage and minimal bias
Comprehensive coverage of all gene sequences and unprecedented low bias expand range of downstream applications.

Accuracy
Use of enzyme with very high proofreading capability yields amplified DNA true to original sequence, enabling high-performance assays such as SNP, genotyping.

Scalability
Larger quantities of DNA generated by increasing reaction volume, enables large-scale studies such as high-density genotyping.

Sample prep steps eliminated
MDA method is carried out directly on crude lysed cells, whole blood, buccal swabs, and other biological samples, eliminating costly, time-consuming DNA purification steps.

Assay-ready
Contaminants effectively diluted out, replacing need for DNA purification and allowing downstream applications to be performed with no inhibitory effects.

High throughput
Ideally suited for 96-well-plate automated systems with no centrifugation and/or filtrations needed.

Long fragments
DNA generated in excess of 10kb in length, enabling molecular biology methods such as RFLP.
VITAL CHARACTERISTICS:
Sample Targets
  • Genomic DNA, whole blood, buffy coats,
    buccal swabs, mouthwash, and cultured cells
Amount
  • 1µl blood, single cheek swab, sub-ng gDNA
Lysis Procedures
  • 10-minute one-step lysis
Yield
  • 50µg DNA from a few genomic copies
    in a 100µl reaction
  • Scalable to 10mg DNA yield from
    large-scale reaction
  • Consistent yield independent of amount
    of starting sample
Reaction Time
  • 6 hours or overnight
Coverage
  • All loci represented at approximately same level
    as in starting genomic DNA target, no more than
    6-fold difference
  • Only highly repetitive sequences lost, such as
    centromere and telomere repeats
Assay-ready DNA
  • PCR-based assays, such as SBE, TaqMan,
    restriction enzyme digestion, and STRs
  • Southern analysis, including RFLP
  • DNA sequencing

TaqMan is a registered trademark of Roche Molecular Systems

For more information on our whole genome amplification product and service portfolio please e-mail us at WGA@MolecularStaging.com.
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