RCAT^TM Advantages

Rolling Circle Amplification Technology (RCAT) is a proprietary amplification technology that offers multiple advantages to help meet the emerging needs of life sciences industries.

RCAT: First-time performance advantages

Expanded multiplexing for protein microarrays
RCAT enables an unprecedented combination of sensitivity and multiplexing advantages for protein microarrays. This allows researchers for the first time to take advantage of the efficiency, throughput, and utility of microarrays for protein detection and measurement.

Unparalleled sensitivity and, in turn, enhanced clinical utility for immunoassays
For the first time sub-pg/mL sensitivities are available for protein analysis, offering scientists significantly improved immunoassays able to detect important biological markers for cancer and infectious disease in concentrations too low to be detected by conventional assays.

An amplification method compatible with today's cellular analysis requirements
For the first time, amplification delivers increased sensitivity and multiplexing capability in immunohistochemistry, in-situ hybridization, and flow cytometry without destroying morphological context. Molecular information can be derived directly from a natural biological setting, within cells and tissues. In addition, targets can be pinpointed in their exact cellular geography. Clinicians and researchers can now access greater information for diagnosis, prognosis, target validation and monitoring.

RCAT: More reliable scientific data — more quickly, consistently, and cost-effectively.

Sensitivity
RCAT achieves an unprecedented increase in sensitivity over conventional protein and nucleic acid assay methods, enabling earlier detection of disease, new proprietary tests, and an enhanced understanding of the role each protein or nucleic acid plays in disease and drug response.

Multiplexing
With RCAT, the signal remains tethered to the analyte or target of interest, enabling simultaneous measurement of many different targets. This addresses the growing interest in protein expression, as well as the trend to diagnose disease with multiple biological markers.

Isothermal environment
The isothermal nature of RCAT ensures that protein function and critical cellular morphology information are preserved. Additionally, the convenience and cost-savings of isothermal technology support the trend toward point-of-care diagnostics.

Cost-effectiveness
Cost advantages of RCAT over current technologies include the minimal use of expensive reagents and precious patient samples, the elimination of time-consuming and expensive thermal cycling, and labor savings achieved through the unique ability of RCAT to enable technology and workstation consolidation for DNA, RNA, and protein applications.