Archive for August, 2009
Methods for DNA sequencing
August 30th, 2009
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Liquid gold organisms
Not many people get organic substances in pure form (or in the form of homogeneous mixtures) are already in antiquity. Fats and oils, no doubt, are among their number. Therefore, their names are in all languages simple native, although the chemists call them also lipids (from the Greek "liposome" - fat). To detect them was easy because they occur in all living organisms, and often in large numbers. Nature took care of animals and plants, providing them with supplies of fuel for cold and hungry days. Animals put this fuel, mainly around the internal organs and under the skin to fat deposits at the same time served as a shock-absorbing cushion shocks and heat insulator. OtherHow to see DNA
Heredity, genes, DNA … It seems that these words have long ceased to be scientific terms, entered into everyday life and is now familiar to every senior, not to mention the students. But there was no DNA, most of us have never seen, though to see it - it is quite real, even in the home. In one of the genetic laboratory on the wall, for example, that the instruction:
- Find something that contains a lot of DNA. For example, green peas (but may be chicken liver, herring roe, or onion).
- Put in a blender about 100 ml (half a cup) of this product, add 1 / 8 teaspoon of salt and 200 ml (cup) of cold water. Whips within 15 seconds. Mixer «cook» you pea-cell soup.
- Strain the mixture through a strainer or a piece of nylon (stockings quite fit). In the obtained pulp, add 1 / 6 of its number (it will be about 2 tablespoons) of liquid detergent (for dishes, for example) and stir well. Leave on for 5-10 minutes.
- Pour the liquid in test tube or other glass vessel, so that each was filled with no more than a third volume.
- Add to each tube for a bit, or the juice squeezed out of a pineapple, a contact lens solution and gently shake it, turning and bending the tube (if you shake too vigorously, break up DNA and did not see it).
- Tilt the tube and slowly pour into it a little bit of ethyl alcohol, that it formed a layer on top of pea mixture. Leite, while a mixture of alcohol and not be a tie. DNA pops up in the form of flakes.
- Wooden stick (pencil) to catch them and examine under a microscope.
Human skin analysis
Human skin is a very complicated structure, constructed from various types of chemicals. It is an integrated system with interacting with each other chemical components. Human skin in 69-73% of water.
Different layers of the skin vary considerably in its content: the share of the epidermis is about 10%, papillary layer - 71-72%. net dermis - about 61%. Normal cells activity is under constant ionic composition and pH of body fluids. In the water, readily soluble chemical compounds containing polar groups and capable of entering into the dipole-dipole interactions with water molecules or form with hydrogen bonds (-OH,-NH, C = 0): non-polar hydrocarbon molecules poorly or not soluble in it.
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RCATTM Technology Details
RCAT is a highly sensitive and efficient amplification method that has been configured in a number of formats to address a broad range of applications.
Linear RCAT (L-RCAT)
One end of a DNA probe is joined to the desired target.
The other end is hybridized to a generic DNA circle.
An enzyme catalyst is added. Starting at the probe for the circle, the enzyme catalyst makes a copy of the DNA circle, returns to the starting point and continues to copy the circle.
A single product is thus generated that is composed of copy after copy of the circle like a thread unraveling from a spool.
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Business development
MSI will build broad usage of the RCAT™ platform and other core technologies through the development of superior diagnostic and genomics tools. To accomplish this, we will pursue vigorous internal development and the establishment of strategic collaborations and licensing agreements to popularize the company’s technology base.
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Employment Opportunities at MSI
Working in a stimulating scientific environment, MSI employees will play a pivotal role in producing a new generation of tests and products with the potential to revolutionize clinical diagnostics as well as genetic analysis. With an emphasis on teamwork, creativity and intellectual achievement, we are dedicated to providing a challenging and stimulating equal opportunity work environment.
We seek experienced and motivated Research Scientists and Research Associates with training in the areas of Immunoassay Development, Microarray & Robotics, DNA Diagnostics & In Situ Hybridization, Protein & Nucleic Acid Chemistry, and Molecular Biology.
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Paper published in Genome Research describes a procedure for rapid amplification of circular DNA developed by Molecular Staging Inc. and Amersham Pharmacia Biotech
New Haven, CT August 13, 2001– Molecular Staging Inc., today announced that, together with Amersham Pharmacia Bio-tech, the two companies have developed a simple method of using Rolling Circle Amplification to copy circular DNA for sequencing, 10,000 fold in a few hours.
“This procedure, which removes the need for lengthy growth periods and traditional DNA isolation procedures, will deliver operational efficiency gains for DNA sequencing centers and genomics companies” said Dr. Stephen Kingsmore, COO of Molecular Staging Inc.
The method is very simple, involving only a few steps, namely heat lysis of the organism containing the DNA of interest and an isothermal amplification step.
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Molecular Staging Inc updates Motorola Inc.’s intended usage of Rolling Circle Amplification Technology.
New Haven, CT. June 4, 2001 - Molecular Staging Inc. (MSI) today confirmed that Motorola will not exercise an option to license Rolling Circle Amplification technology (RCAT™) for applications in the clinical diagnostics market. Instead, Motorola Inc. intends to pursue the use of RCAT™ for a number of research applications.
Motorola Inc. and MSI entered into a license option agreement related to the use of RCAT™ on clinical diagnostic microarrays, November 7, 2000, whereby Motorola Inc. paid certain research • fees and made an equity investment in MSI. The agreement provided for an evaluation period during which Motorola Inc and MSI would work together to meet specific technical milestones.
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Molecular Staging Completes $41.25 Million Private Financing
Funding will enhance Molecular Staging’s leadership position in signal and target amplification and accelerate MSI’s efforts in the proteomics, genomics and molecular diagnostics markets.
NEW HAVEN, CT — Molecular Staging Inc. (MSI), a privately-held life sciences tool company, announced today that it has completed a Series D preferred stock financing totaling $41.25 million. Investor AB, through Investor Growth Capital, participated in the private placement along with OrbiMed, CIBC Capital Partners and Cooper Hill Partners. Domain Partners III, CHL Medical Partners and 3i Bioscience Investment Trust, who were already holders of preferred stock in Molecular Staging, also participated in the new round of financing. Investor AB and OrbiMed will be entitled to name two representatives to the board.
Liza Page Nelson, Managing Director of Investor Growth Capital said “Molecular Staging has made tremendous progress in the development and validation of its key platforms. Seldom does one find a company whose technology can broadly address so many markets; these include research reagents, drug research and development, diagnostics, and life sciences.”
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